Fig. 6: The unique nucleotide-binding pocket of AsfvAP.
From: A unique DNA-binding mode of African swine fever virus AP endonuclease
a The H-bond interactions maintained by the R3 region residues in the AsfvAP/DNA-1 complex. DNA and the R3 region are shown as stick and cartoon, respectively. The C atoms of DNA and the R3 region residues are colored in yellow and green, respectively. b Superposition of DNAs and the R3 regions observed in the AsfvAP/DNA-1 and EcNfo/substrate complexes. AsfvAP/DNA-1 complex is colored as in a, the C atoms are colored in blue for both DNA and the R3 region in the EcNfo/substrate complex. c Surface presentation showing the nucleotide-binding pocket of AsfvAP. d Comparison of DNA binding by WT AsfvAP and mutant proteins. e, f SDS-PAGE gel analysis and comparison of DNA-3 cleavage by WT and mutated AsfvAP. The substrate and product bands are labeled by S and P, respectively. In panels d, f, the data represent the mean of three independent experiments. The standard deviation (±SD) values are indicated by error bars. All SDS-Page gel analysis were repeated for at least three times, the representative gels were shown in panels e.
