Fig. 5: Binding of REF6 to different dsDNA fragments.
From: Crystal structures of REF6 and its complex with DNA reveal diverse recognition mechanisms
a EMSA of REF6 with single-base mutations of the NAC004 fragment. b MST measurement of the binding affinity of m12 (mutated G:C base pair with an A:T base pair) with REF6. c Modeling of the m12 and REF6 interaction. The mutation from C9′ (left) to T9′ (right) creates a steric clash with REF6. REF6 can bind to both CUC2 (d) and CUC1 (e) dsDNA fragments based on EMSA. f Comparison of the binding affinities of CUC1 and CUC2 with REF6. Note that CUC1-3 + 4 has two CTCTGYTY motifs.
