Fig. 4: In vitro expansion of rat islet clusters.
From: In vitro expansion of pancreatic islet clusters facilitated by hormones and chemicals
a Representative images of rat islet single cells or clusters (~5000–8000 µm2 original surface area) at various time points after 3D culture with PIEM. The scale bar in the single-cell image represents 50 µm, and one in the cluster image represents 82.5 µm. b Numbers of expanded clusters per 100 islet cell clusters. The experiments were performed on rats in triplicate. The data are shown as the mean ± SEM. c Comparison of the expression of islet cell identity markers in rat-expanded clusters compared with primary islets. *P < 0.05; **P < 0.01; clusters were compared with primary rat islets. The data are shown as the mean ± SEM of at least three independent experiments. Data were analysed using unpaired two-tailed Student’s t-test.
