Fig. 6: Expanded BCR clones and biased usage of VDJ genes observed in the COVID-19 patients.
From: Immune cell profiling of COVID-19 patients in the recovery stage by single-cell sequencing
a The UMAP plot shows the B cell expansion status in the HCs and the ERS and LRS COVID-19 patients. b The bar plots show the clonal expansion status of B cells in peripheral blood from each individual sample. The number of color blocks represents the complexity of the clonal states. c Separate analysis of HC, ERS and LRS group by percentages of maximum clones revealed an enrichment of highly expanded clones (defined as comprising 10% or more of all BCR sequences; indicated by dotted line) in each group. None of healthy subjects had a highly expanded clone, versus four out of five patients in ERS, one out of five patients in LRS. Values are mean ± SD. d The volcano plot shows the DEGs of expanded vs. non-expanded B cells in ERS and LRS patients. P values were calculated using a paired, two-sided Wilcoxon test and FDR corrected using the Benjamini–Hochberg procedure. e The bar plots show specific IGHV, IGKV, IGLV usage in the HCs and the ERS and LRS COVID-19 patients. f Heatmap showing IGH rearrangements in peripheral blood samples from ERS group.
