Fig. 3: Softness facilitates M1 AMs to take up viruses.
From: Distinct uptake, amplification, and release of SARS-CoV-2 by M1 and M2 alveolar macrophages
a AMs isolated from WT or hACE2 mice were infected with SARS-CoV-2 for 2 h. The viral load was analyzed by qPCR. b The virus load from ACE2-siRNAs AMs was determined by qPCR. c The stiffness of M0, M1, and M2 AMs was measured by atomic force microscopy. d M1 or M2 AMs were treated with jasplakinolide (Jas, 50 nM) or cytochalasin D (Cyto, 1 μM) for 12 h or 4 h, respectively. Then, AMs were infected with SARS-CoV-2 for 2 h and the viral load was determined by qPCR. The data represent the mean ± SD of 3 independent experiments. **P < 0.01, ***P < 0.001, by one-way ANOVA (a, b and d) or Kruskal–Wallis test (c).
