Fig. 4: The pH value in endosome or lysosome affects the viral replication in AMs.
From: Distinct uptake, amplification, and release of SARS-CoV-2 by M1 and M2 alveolar macrophages
a M0, M1, and M2 AMs were stained with pHrodo™ Red dextran and the fluorescence intensity was determined by flow cytometry. b, d AMs were infected with SARS-CoV-2 for 30 min (b) or 1 h (d), and then stained with anti-NP, anti-Rab7, and anti-lamp2 antibodies. Scale bar, 5 μm. c M0, M1, and M2 AMs were stained with LysoSensor™ Yellow/Blue DND-160 and the pH value was detected by a microplate reader. The data represent the mean ± SD of 3 independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, by one-way ANOVA (a–d).
