Fig. 2: Expression of specific development-related markers.
From: Generation of human blastocyst-like structures from pluripotent stem cells
a–f Immunofluorescence staining of human EPS-blastoids for EPI lineage marker (OCT4), TE lineage markers (CK8 and GATA2/3), and PE lineage marker (GATA6), Scale bar = 50 μm. g Model of immunofluorescence staining of human blastocyst for OCT4 in EPI, GATA6 in PE, GATA2/3, and CK8 in TE lineages, Scale bar = 50 μm. h–j Quantification of the percentage of human blastoids with correct allocation of OCT4 and CK8 (h), GATA6 (i), and all three markers (j). Data in i, data are means ± SD (n = 172 blastoids). *P < 0.05. Data in i, j, data are means ± SD (n = 131 blastoids).
