Fig. 2: Liver-specific SIRT2 KO sensitizes mice to alcoholic liver injury.
From: SIRT2-mediated deacetylation and deubiquitination of C/EBPβ prevents ethanol-induced liver injury
SIRT2f/fAlb-Cre– (LoxP) and SIRT2f/fAlb-Cre+ (SIRT2-KO) male mice were treated with pair (Pair) and ethanol diet (EtOH) according to NIAAA model (n = 8–10/group). a–h Liver injury, steatosis, lipid peroxidation, and cell apoptosis were assessed by images of the indicated livers (scale bar, 1 cm), mouse hepatic H&E staining (scale bar, 100 μm), IHC detection of 4-HNE and TUNEL (scale bar, 100 μm) (a), serum ALT (b) and AST (c), liver triglyceride (TG) (d), hepatic MDA content (e), and PTGS2 mRNA (f), quantitative analysis of TUNEL-positive hepatocytes (magnification, ×200) (g), Western blot analysis of cl.Caspase-3 in murine liver tissues (h). Student’s t-test was used for statistical evaluation. Data are shown as means ± SD and are considered statistically significant at *P < 0.05, **P < 0.01, and ***P < 0.001.
