Fig. 3: Hepatocyte-specific SIRT2 overexpression prevents alcoholic liver injury in mice.
From: SIRT2-mediated deacetylation and deubiquitination of C/EBPβ prevents ethanol-induced liver injury
Male C57BL/6 mice tail injected with AAV8-Ctrl (Ctrl), AAV8-SIRT2 (SIRT2), or AAV8-SIRT2-H187A (H187A) were treated with pair (Pair) and ethanol diet (EtOH) according to NIAAA model (n = 7/group). a Images of the indicated livers (scale bar, 1 cm), murine liver staining by H&E (scale bar, 50 μm), IHC detection of 4-HNE and TUNEL assay (scale bar, 100 μm). b–d Serum ALT (b) and AST (c) measurements and TG content (d). e–f Hepatic MDA measurements (e) and PTGS2 mRNA analysis by qRT-PCR (f). g Quantitative analysis of TUNEL-positive hepatocytes (magnification, ×200) in murine livers. h Western blot analysis of cl.Caspase-3 and SIRT2 expression in murine liver tissues. Student’s t-test was used for statistical evaluation. Data are shown as means ± SD and are considered statistically significant at *P < 0.05, **P < 0.01, and ***P < 0.001.
