Fig. 2: HBV induces significant changes in 35 DEGs at both transcriptional and translational levels (ttDEGs) with PGAM5 and SIRT6 emerging as potential host virus-restricting factors. | Cell Discovery

Fig. 2: HBV induces significant changes in 35 DEGs at both transcriptional and translational levels (ttDEGs) with PGAM5 and SIRT6 emerging as potential host virus-restricting factors.

From: Multiomics interrogation into HBV (Hepatitis B virus)-host interaction reveals novel coding potential in human genome, and identifies canonical and non-canonical proteins as host restriction factors against HBV

Fig. 2

a Experimental approaches for transcriptomic and translatomic profiling of host genes upon transfection with the Cre-based rcccDNA system of HBV. b The cumulative TE distribution among HBV groups and control groups, host genes were translated less efficiently in HBV groups than that in control groups. P-values for comparison between each of the HBV and control group were calculated using Kolmogorov–Smirnov test with the results shown on the top. c Heatmap and d GO annotation of the 35 ttDEGs, whose expression was significantly altered by HBV in both transcription and translation. e RT-qPCR reveals that HBV downregulated the transcriptional level of endogenous PGAM5 and upregulated PPP1R15A transcription. f, g Reinstatement of the static levels of two representative ttDEGs affected the expression of cellular HBcAg (f) and secreted HBsAg and HBeAg (g). The major bands were marked with asterisks to indicate the theoretical molecular weights of the ectopically expressed ttDEGs. *P < 0.05, **P < 0.01, ***P < 0.001. ELISA data are presented as bar chart (n = 3); qPCR results are presented as bar chart (n = 3).

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