Fig. 6: MDL-800 restricts HBV in vitro and in vivo.

a, b HepG2-NTCP cells were infected either with vehicle (mock) or HBV particles purified from the culture medium of HepAD38 cells, the HBV groups were treated with DMSO or MDL800, and the HBeAg (a) and HBsAg (b) level in culture medium of each group were collected and measured at indicated time points. c A Mouse model for HBV infection was established as previously described²⁵. Peripheral blood samples were collected and subjected to ELISA to detect serum HBsAg, for vehicle group and MDL800 group. d Peripheral blood samples of mice treated with MDL800 or vehicle were collected and subjected to Alanine-Aminotransferase (ALT) assay. e HE or immunohistochemistry staining of liver sections from mice receiving vehicle or MD800 from experiment in F. Representative images of indicated group were shown. For the panel labeled H&E and H3K56ac, Scale bars, 200 μm; For the panel labeled HBcAg, Scale bars, 100 μm. f Statistics analysis of IHC results of indicated group, the mean integral optical density (IOD mean) of five random visual fields for each sample (n = 9 for vehicle group and n = 10 for MDL800 group) was measured. g Summary of the major findings in this study. Multiomics interrogation into HBV-host interaction has led to the discovery of multiple HBV-induced DEGs including canonical and non-canonical genes in host cells. a, b Two-way ANOVA test was used, for others, student t-test was used. *P < 0.05, **P < 0.01, ***P < 0.001.