Fig. 5: Deletion of rs9311399-associated enhancer leads to alteration in cancer-related pathways and tumorigenic capacity. | Cell Discovery

Fig. 5: Deletion of rs9311399-associated enhancer leads to alteration in cancer-related pathways and tumorigenic capacity.

From: Genome-wide association and functional interrogation identified a variant at 3p26.1 modulating ovarian cancer survival among Chinese women

Fig. 5

a Volcano plot of RNA-seq for differentially expressed genes between OVCA432 WT and KO cells. b, c Pathways enrichment analysis of RNA-seq differentially expressed genes using KEGG and Reactome gene sets. d, e The representative images showed colony formation assay. Deletion of rs9311399-associated enhancer reduced colony formation in OVCA432 WT and KO cells. f siRNA-mediated knockdown of BHLHE40-AS1 attenuates cell proliferation in both OVCA432 and SKOV3 cells. Statistical comparisons of colony numbers or cell proliferation were undertaken using Student’s t tests. Data are shown as mean ± sd with **P < 0.01, ***P < 0.001.

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