Fig. 6: HAT1 regulates H4K5mea in cells. | Cell Discovery

Fig. 6: HAT1 regulates H4K5mea in cells.

From: Histone lysine methacrylation is a dynamic post-translational modification regulated by HAT1 and SIRT2

Fig. 6

a Close-up view of the HAT1-acetyl-CoA interaction site in the crystal structure of HAT1 in complex with acetyl-CoA and histone H4 peptide (PDB ID: 2P0W). HAT1 is shown in cartoon representation in gray, acetyl-CoA in green, and histone H4 in yellow. b Close-up view of the methacrylyl-CoA interaction site with HAT1 in a model generated by fitting the methacrylyl-CoA into the isobutyryl-CoA electron density map in the crystal structure of HAT1-isobutyryl-CoA (PDB ID: 6VO5) for illustration purposes. HAT1 is shown in cartoon representation in gray, methacrylyl-CoA in cyan and histone H4 in yellow. c HEK 293 T were transfected with empty pcDNA 3.1 vector or pcDNA 3.1 HAT1-Flag plasmid for 48 h before harvesting. Cell lysates were prepared using RIPA buffer and then analyzed by western blot. d HEK 293 T cells were transfected for 72 h with 25 nM of control or HAT1 siRNA. RIPA lysates were probed using indicated antibodies. e MEF HAT1−/− and MEF HAT1+/+ cells were grown in culture before harvesting lysates. RIPA lysates were assessed by western blot as indicated.

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