Fig. 1: scRNA-seq reveals cellular heterogeneity in the thoracic aorta during the development of TAAD. | Cell Discovery

Fig. 1: scRNA-seq reveals cellular heterogeneity in the thoracic aorta during the development of TAAD.

From: Single-cell RNA sequencing identifies an Il1rn+/Trem1+ macrophage subpopulation as a cellular target for mitigating the progression of thoracic aortic aneurysm and dissection

Fig. 1

a Schematic representation of the scRNA-seq procedure. The ascending aorta and aortic arch were collected at three time points during the induction, i.e., after 7, 14, and 21 days of BAPN or vehicle (drinking water) administration. Tissues from six mice were pooled for each time point for scRNA-seq. CTRL, control. b Unbiased clustering of 61,826 cells from all six samples reveals 24 cellular clusters. The number in the parenthesis indicates the cell count. c Hierarchical clustering of the clusters based on the average expression of the 2000 most variable genes. d Expression of the established marker genes specific for each lineage in each cluster. e Representative molecular signatures for each cell cluster. The area of the circles indicates the proportion of cells expressing the gene, and the color intensity reflects the expression intensity. EDO, endothelial cell; FB, fibroblast; MAC, macrophage; PCD, pericardial cell; SMC, smooth muscle cell.

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