Fig. 2: Endogenous transcription factors regulate gene activation/inactivation.
From: CRISPR signal conductor 2.0 for redirecting cellular information flow
a Schematic diagram showing the molecular mechanism underlying enhanced transcription of cellular genes accomplished by binding endogenous transcription activators. b The effect of gene activation was determined by dual-luciferase reporter assay. Relative luciferase activities were determined as the ratios between Rluc and Fluc values. c The relative expression levels of P21 and MALAT1 upregulated by endogenous transcription activators were determined by real-time quantitative PCR (RT-qPCR). d Schematic diagram showing the molecular mechanism leading to repressed transcription of cellular genes by binding endogenous transcription repressors. e The effect of gene inactivation was detected by the dual-luciferase reporter assay. f The relative expression levels of p21 and MALAT1 were downregulated by endogenous transcription repressors, compared to the control, as determined by RT-qPCR. The results are shown as the means ± SD. Each experiment was performed in triplicate for five independent times. *P < 0.05; **P < 0.01, compared to the control, two-tailed t-test.
