Fig. 8: Identification of critical binding residues for 2G1. | Cell Discovery

Fig. 8: Identification of critical binding residues for 2G1.

From: Broad ultra-potent neutralization of SARS-CoV-2 variants by monoclonal antibodies specific to the tip of RBD

Fig. 8

a Statistics of mutation proportion in RBD residue 471Glu–490Phe where key for 2G1 epitope from GISAID database as of August 2021. b Identification of critical binding residues for 2G1. Spike genes with high frequency mutation sites between 471Glu and 490Phe (> 0.05%) were cloned and transiently expressed on the surface of 293T cells. The binding ability of 2G1 to these mutant S proteins was measured by flow cytometry. The fold change of binding ability was normalized by comparing to WA1/2020S protein. c Mutations in the key interaction sites of 2G1 that affect the binding ability of 2G1 to varying degrees.

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