Fig. 4: The neutralizing antibody responses against 23 various SARS-CoV-2 pseudo-virus strains elicited by mutI-tri-RBD were significantly stronger than or comparable to those induced by homo-tri-RBD.
From: Design of a mutation-integrated trimeric RBD with broad protection against SARS-CoV-2
a Sensitivities to the neutralization of homo-tri-RBD immunized sera for the various pseudo-typed variants compared with that of the prototype strain. The sera collected on day 14 post-immunization (D35 in Fig. 2a) were used in the pseudo-virus neutralization assays. In this figure, the titers of the prototype strain are taken as a reference, and the titer ratios between the variants and the prototype strain are displayed. Each serum sample is presented as a dot in the plot, and each serum sample was tested against all these variants. Data are presented as means ± SEM. P values were calculated with Student’s t test. *P < 0.05, **P < 0.01. b Neutralizing antibody GMTs against the various pseudo-typed strains induced by mutI-tri-RBD (red color) compared with those elicited by homo-tri-RBD (blue color). Numbers in the red box indicate the GMT ratios of mutI-tri-RBD to homo-tri-RBD. Data are presented as means ± SEM. P values were calculated by using one-way ANOVA followed by Sidak’s multiple comparison test. *P < 0.05, **P < 0.01.
