Fig. 4: CD44 enhanced cell rigidity via RhoA and Rac1.
From: Cell-in-cell structure mediates in-cell killing suppressed by CD44
a CD44 promoted cell internalization. Orange and gray represented the internalized cells with relatively high and low CD44 expression, respectively. EV: empty vector, NC: non related control. Data are means ± SD of more than three images of 20× objective. n > 59/each. b Representative images showing homotypic CIC structures with CD44-high cells internalized (in red and green, respectively). Scale bar, 20 µm. c CD44 positively regulated the expression of RhoA and pMLC in PLC/PRF/5 isogenic cells. d Time-lapse images in x–z plane for the deformation of PLC/PRF/5 isogenic cells compressed by agarose gel. e, f CD44 regulated cellular deformation dynamics over time (e), or in response to different weight loads (nN) (f) as determined by agarose compression assay. Data are means ± SD of triplicate experiments. g CD44 enhanced cell rigidity via RhoA and Rac1 as determined by agarose compression assay. Data are means ± SD of triplicate experiments. h The siRNA-mediated knockdown of Rac1 and RhoA in A4S and F6ft cells, respectively. i Formation of heCIC structures were inhibited, or promoted by knocking down Rac1 or RhoA, respectively, in PLC/PRF/5 isogenic cells. Data are means ± SD of more than three images of 20× objective. n > 300 cells analyzed for each clone. *P < 0.05; ***P < 0.001. j Overexpression of RhoA and Rac1in A4S and F6ft cells, respectively. k Formation of heCIC structures were inhibited, or promoted by overexpressing RhoA or Rac1, respectively, in PLC/PRF/5 isogenic cells. Data are means ± SD of more than three images of 20× objective. n > 300 cells analyzed for each clone. ***P < 0.001.
