Fig. 6: Cytotoxic stimuli facilitates the release of degraded RNA m6A nucleoside to extracellular fluid and induces the misincorporated DNA 6 mA in neibourghing cells. | Cell Discovery

Fig. 6: Cytotoxic stimuli facilitates the release of degraded RNA m6A nucleoside to extracellular fluid and induces the misincorporated DNA 6 mA in neibourghing cells.

From: Rare and misincorporated DNA N6-methyladenine is a hallmark of cytotoxic stresses for selectively stimulating the stemness and proliferation of glioblastoma cells

Fig. 6

a, b The release of m6A (a) or rA (b) nucleoside from glioblastoma cells after H2O2 treatment. Glioblastoma cells were treated for 24 h with or without 1 mM H2O2, and then supernatants were collected and subjected to LC-MS/MS analysis. c Flow diagram of stable isotope labeling for tracing genomic incorporation. d Quantification of genomic DNA [D3]-6mA in in glioma cell lines, GSCs, and glial cell line. The cells were treated with [D3]-m6A at the concentration as indicated. (◊ indicated ND (not detected)).

Back to article page