Fig. 1: rRNA associates with chromosomes during mitosis.
From: Ribosomal RNA regulates chromosome clustering during mitosis
a A schematic diagram shows the procedure of RNA purification from soluble and pellet fractions of metaphase cells. b Purified RNA is analyzed by agarose gel. c Distribution of RNA-seq reads (CPM) across the full-length 45S pre-rRNA sequence in soluble and pellet total RNAs. d Northern blot analysis of the RNAs from the soluble (S) and pellet (P) fractions with probes targeting 18S, ITS1, 5.8S, ITS2, and 28S regions. The position of northern blot probes is shown in c. e The ratio of RNA in the pellets to total DNA mass (left y axis) and the ratio of RNA in the pellets to total RNA (right y axis).
