Fig. 4: Ki-67 mediates pre-rRNA’s association with chromosomes.
From: Ribosomal RNA regulates chromosome clustering during mitosis
a The protein species in the pellet fraction. The protein species was isolated from TRIzol extraction and analyzed by mass spectrometry. The top six abundant proteins are shown. b The localization of Ki-67 and pre-rRNA at the periphery of chromosomes. Pre-rRNA was examined by RNA FISH with probes against the ITS1 region in mitotic cells treated with or without BMH-21. Ki-67 was examined with anti-Ki-67 antibody. Signal intensity of pre-rRNA, Ki-67, and DAPI at the indicated section was analyzed (right panel). Scale bar, 5 μm. c The ratio of RNA in the pellets to total DNA mass (left y axis) and the ratio of RNA in the pellets to total RNA (right y axis) in parental 293T and Ki-67 knockout cells. **P < 0.01. d Localization of pre-rRNAs detected by RNA FISH with Cy5-labeled probe against 5’ETS, FAM-labeled probes against ITS1 and Cy3-labeled probe against 28S region of pre-rRNAs at the perichromosomal layer in parental 293T and Ki-67-knockout cells. Scale bar, 10 μm. e The RNA FISH signals were analyzed by “Local Thickness” of Fiji, the dashed square shows the region of interest. ***P < 0.001.
