Fig. 6: Pre-rRNA facilitates chromosome dispersion at prometaphase.
From: Ribosomal RNA regulates chromosome clustering during mitosis
a Ki-67 promotes chromosome dispersion. Chromosome areas at prometaphase were measured in the cells expressing full-length Ki-67 or indicated Ki-67 mutants. b RNA polymerase I inhibitor treatment suppresses chromosome dispersion. Parental 293T cells or Ki-67-deficient cells were treated with BMH-21. The localization of Ki-67, pre-rRNA, and NOL1 was examined. Scale bar, 5 μm. c Chromosome areas were analyzed by ImageJ. *P < 0.05; **P < 0.01; ***P < 0.001. d A schematic model shows that pre-rRNAs gradually translocated from nucleoli to the periphery of chromosomes during mitosis. Ki-67 anchors these rRNA species on the condensed chromosomes.
