Fig. 1: Transcriptomic landscape of hematopoietic cells in human G-BM. | Cell Discovery

Fig. 1: Transcriptomic landscape of hematopoietic cells in human G-BM.

From: Decoding lymphomyeloid divergence and immune hyporesponsiveness in G-CSF-primed human bone marrow by single-cell RNA-seq

Fig. 1

a Workflow showing the processing of paired unstimulated BM and G-CSF-primed BM (G-BM) samples from healthy donors for scRNA-seq. b Uniform manifold approximation and projection (UMAP) visualization of main lineages in human BM based on single-cell transcriptomes. Each dot represents a single cell; colors indicate cell clusters with numbered labels. c UMAP visualization of the expression of lineage-specific marker genes (top) and activity of canonical cell type-specific regulons (bottom) in identified cell lineages. d Bar plot showing the changes in the percentage of each cell lineage upon G-CSF treatment. Pre, representing samples before G-CSF treatment; Post, representing samples after G-CSF treatment. e Dot plot showing average expression of top 10 DEGs for hematopoietic cells in unstimulated BM and G-BM and the percentage of cells expressing the genes. DEGs were detected using function FindAllMarkers in Seurat (Wilcoxon-rank-sum test, with P value adjusted for multiple testing using Benjamini–Hochberg correction). f Bar plots from the Metascape analysis showing the major enriched terms of DEGs before (top) and after (bottom) G-CSF administration. The length of each bar represents –log10(P value).

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