Fig. 2: The effect of G-CSF on hematopoietic differentiation in human BM. | Cell Discovery

Fig. 2: The effect of G-CSF on hematopoietic differentiation in human BM.

From: Decoding lymphomyeloid divergence and immune hyporesponsiveness in G-CSF-primed human bone marrow by single-cell RNA-seq

Fig. 2

a UMAP visualization of subclusters in HSPCs. Each dot represents a single cell; colors indicate clusters (top) and sources of samples (bottom). HSC hematopoietic stem cell; MPP multipotential progenitor; LMPP lymphoid-primed multipotent progenitor; CLP common lymphocyte progenitor; Pro-B progenitor B cell; GMP granulocyte-monocyte progenitor; MEP megakaryocyte/erythroid progenitor; ErP erythroid progenitor; MkP megakaryocyte progenitor. b Heatmap displaying scaled expression of canonical cell type-associated genes for clusters in a. Color scale corresponds to z-scored, log-transformed mean gene-expression counts for each cell state. c Heatmap showing scaled expression of top 3 DEGs for clusters in a. Color scale corresponds to z-scored, log-transformed mean gene-expression counts for each cell state. Detailed DEGs can be found in Supplementary Table S2. d Bar plot showing the changes in the percentage of subclusters of HSPCs upon G-CSF treatment. e Cell proportion of each subcluster within HSPCs derived from BM grafts before (left) and after (right) G-CSF administration. f Monocle prediction of HSPC developmental trajectory with cluster information (left) and pseudotime (right) mapped. g Density plots displaying the distribution of different hematopoietic progenitor cells in trajectories before (left) and after (right) G-CSF administration. These plots were based on the 2D kernel density estimation. The density of lines and intensity of colors are proportional to cell density in each state.

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