Fig. 6: Indirect modulation of G-CSF on T and NK cells. | Cell Discovery

Fig. 6: Indirect modulation of G-CSF on T and NK cells.

From: Decoding lymphomyeloid divergence and immune hyporesponsiveness in G-CSF-primed human bone marrow by single-cell RNA-seq

Fig. 6

a Sankey plot showing the upregulated intercellular communication between the potential sender cells and effector CD8+ T cells upon G-CSF administration. Differential intercellular communication analysis was performed using R package scDiffCom. Ligand–receptor pairs with adjusted P value < 0.05 and fold change > 1.5 are displayed. b Plot showing regulation of top predicted target genes in effector CD8+ T cells mediated by upregulated ligand–receptor pairs between effector CD8+ T cells and potential sender cells. Transcription factors in target genes are represented as diamonds. The ligand–receptor–target relationship was obtained from the database containing ligand–receptor interactions and receptor–transcription factor interactions in R package scMLnet, and database including ligand–receptor interactions and ligand–target links in R package NicheNet. c Heatmap showing the average expression of the downstream target genes in b in effector CD8+ T cells before and after G-CSF administration. d Sankey plot showing the upregulated intercellular communication between the potential sender cells and NK cells upon G-CSF administration. e Plot showing the regulation of top predicted target genes in NK cells mediated by upregulated ligand–receptor pairs between NK cells and potential sender cells. f Heatmap showing the average expression of the target genes in e in NK cells before and after G-CSF administration.

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