Fig. 3: neCtnnb1 knockout hampered the production and transit-amplification of IPs. | Cell Discovery

Fig. 3: neCtnnb1 knockout hampered the production and transit-amplification of IPs.

From: A Ctnnb1 enhancer regulates neocortical neurogenesis by controlling the abundance of intermediate progenitors

Fig. 3

a–k Immunofluorescence (a) and quantification (c–k) of EdU + (red), PAX6 + (blue) and TBR2 + (green) cells on boxed area of coronal sections (b) of E16.5 WT and neCtnnb1KO Ncx. Pregnant mice were injected with EdU 2 h before sacrifice. Each point represents an individual brain. l–t Immunofluorescence (l) and quantification (n–t) of Ki67 + (red), TBR2 + (blue) and BrdU + (green) cells on boxed area of coronal sections (m) of E16.5 WT and neCtnnb1KO Ncx. Pregnant mice were injected with BrdU at E15.5. m–t Quantification of data in l. Each point represents an individual brain. Quantification data are shown as means ± SEM. Statistical significance was determined using an unpaired two-tailed Student’s t-test (c–k, n–t). *P < 0.05, **P < 0.01, and ***P < 0.001. ns not significant. Scale bars, 100 μm (a and l), 50 μm (magnified views in a, l).

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