Fig. 6: Schematic model of 4H30 inhibiting SARS-CoV-2 infection by triple mechanisms.
From: A trifunctional peptide broadly inhibits SARS-CoV-2 Delta and Omicron variants in hamsters
a 4H30 can cross-link SARS-CoV-2 to form big virus clusters, which can block the two entry pathways in VeroE6 (mediated by endocytosis) and Calu-3 cells (mediated by TMPRSS2). b SARS-CoV-2, which is not cross-linked by 4H30 and enters cells by endocytosis, can be stopped in endosomes due to the inhibition of 4H30 on endosomal acidification, which blocks virus envelope fusion with the endosomal membrane. c 4H30 can stop viral release by cross-linking virus with cell-surface HS/CS. Collectively, 4H30 can inhibit viral entry, fusion, and release of SARS-CoV-2.
