Fig. 1: Single cell RNA-seq profiling and unbiased clustering of cells from full thickness human uterine tissues.
From: SFRP4+ stromal cell subpopulation with IGF1 signaling in human endometrial regeneration
a Workflow shows sample processing, enzymatic digestion and drop-seq based single cell RNA-seq. b UMAP plot of 6 main clusters, donor ID and secretory/proliferative origin phases of full-thickness uterus by single cell RNA-seq (scRNA-seq). c Heatmap shows specifically expressed gene signature of the 6 main clusters of full-thickness uterus. d Gene ontology (GO) analysis of the specifically expressed gene signature of the 6 main clusters of full-thickness uterus. e Violin plot showed specific marker genes from gene signatures of each cell cluster (DES, CNN1 for myometrial smooth muscle cells; COL1A1, ECM1 for stroma cells; KRT8, EPCAM for epithelial cell; CLDN5, VWF for endothelial cells; ADIRF, CRIP1 for vascular smooth muscle cells; PTPRC, CD68 for immune cells). f Immunohistochemistry staining from the HPA further validated the expression of specific markers of the 6 main clusters: COL1A1 (https://www.proteinatlas.org/ENSG00000108821-COL1A1/tissue/endometrium#img), ECM1 (https://www.proteinatlas.org/ENSG00000143369-ECM1/tissue/endometrium#img) was expressed in the uterus stromal cells, DES (https://www.proteinatlas.org/ENSG00000175084-DES/tissue/endometrium#img) was expressed in the uterine myometrial smooth muscle cells, ADIRF (https://www.proteinatlas.org/ENSG00000148671-ADIRF/tissue/endometrium#img) was expressed in the uterine vascular smooth muscle cells, CLDN5 (https://www.proteinatlas.org/ENSG00000184113-CLDN5/tissue/endometrium#img), VWF (https://www.proteinatlas.org/ENSG00000110799-VWF/tissue/endometrium#img) were expressed in the uterus endothelial cells, EPCAM (https://www.proteinatlas.org/ENSG00000119888-EPCAM/tissue/endometrium#img), KRT8 (https://www.proteinatlas.org/ENSG00000170421-KRT8/tissue/endometrium#img) were expressed in the uterus epithelial cells,, PTPRC (https://www.proteinatlas.org/ENSG00000081237-PTPRC/tissue/endometrium#img), CD68 (https://www.proteinatlas.org/ENSG00000129226-CD68/tissue/endometrium#img) were expressed in the uterus immune cells. Scale bar, 25 µm.
