Fig. 1: Upregulation of ACE2 expression on the cell surface of peripheral blood myeloid populations upon ex vivo TLR stimulation. | Cell Discovery

Fig. 1: Upregulation of ACE2 expression on the cell surface of peripheral blood myeloid populations upon ex vivo TLR stimulation.

From: Surface translocation of ACE2 and TMPRSS2 upon TLR4/7/8 activation is required for SARS-CoV-2 infection in circulating monocytes

Fig. 1

a Representative flow cytometry analysis of ACE2 protein expression (red line) in T cells, B cells, CD14+ MO, cDCs, and pDCs from fresh PBMCs isolated from healthy donors. Mouse IgG1 antibody (gray tinted) was used as isotype control. b Frequency of cells expressing cell surface and cytoplasmic ACE2 as in (a) (n = 4 biologically independent samples/group). c PBMCs were cultured ex vivo with or without R484 or LPS for 24 h, followed by flow cytometry analysis of surface ACE2 in CD14+ MO, cDCs, and pDCs. Mouse IgG1 antibody was used as the isotype control. d Frequencies of indicated cell types expressing surface ACE2 as in c (n = 6 biologically independent samples/group). cDC classical dendritic cells, pDC plasmacytoid DCs, MO monocytes, LPS lipopolysaccharide.

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