Fig. 3: Functional characterization of cachexia-specific progenitor cell clusters. | Cell Discovery

Fig. 3: Functional characterization of cachexia-specific progenitor cell clusters.

From: Single-cell sequencing unveils key contributions of immune cell populations in cancer-associated adipose wasting

Fig. 3

a UMAP plot showing the origins of all progenitor cells. b Dot plot showing the enriched biological processes among downregulated genes in vPreAl from patients with cachexia. c Volcano plot showing the differences in the expression of genes involved in fat cell differentiation in vPreA. Red dots denoting genes significantly upregulated in VAT from patients with cachexia, and blue dots denoting genes down-regulated from patients without cachexia. d Boxplot showing the proportion of progenitor cells derived from patients with (n = 4) or without (n = 4) CAC for each cell population. e Bar plot showing significantly enriched KEGG pathways in DEGs between inflammatory progenitor cell and the other progenitor cells in SAT. The color saturation of bars indicating the significance of enrichment. P-values were adjusted by the FDR. f Relative mRNA expression levels of CCL2, CXCL2, and TNFAIP6 in white preadipocytes in each group as quantified by qPCR analyses. P-values were determined by the two-way ANOVA with post hoc Dunnett’s multiple-comparison tests. *P < 0.05; ***P < 0.001 (n = 3 biological replicates). Values are means ± SEM.

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