Fig. 4: iNK cells exhibit tumor-killing activities in vitro. | Cell Discovery

Fig. 4: iNK cells exhibit tumor-killing activities in vitro.

From: Lateral plate mesoderm cell-based organoid system for NK cell regeneration from human pluripotent stem cells

Fig. 4

a Representative images of residual A1847-tdTomato+ tumor cells (Target, T) after 4-h co-culture with iNK cells (Effector, E) (E:T = 5:1). b Flow cytometry analysis of apoptotic A1847-tdTomato+ tumor cells post co-culture with iNK cells (E:T = 2:1) for 1 h and 4 h. n = 6 each group. c Cytotoxicity analysis of the iNK cells. iNK cells were co-cultured with CFSE-labeled A1847 tumor cells for 4 h at the indicated effector to target ratios. n = 6 each group. d ADCC analysis of iNK cells. iNK cells were co-cultured with CFSE-labeled Raji cells in the presence of anti-CD20 antibody (Rituximab, 20 μg/mL) for 4 h at the indicated effector to target ratios. n = 9 each group. Data were collected from two (b, c) or three (d) independent experiments. One-way ANOVA and Kruskal-Wallis tests were used for statistics. NS, not significant. ESC: human ESC line hPSC-2; iPSC: human iPSC line hPSC-6.

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