Fig. 2: Hepatocytes derived from Gli1+ nonhepatocytes upon chronic injury. | Cell Discovery

Fig. 2: Hepatocytes derived from Gli1+ nonhepatocytes upon chronic injury.

From: Identification of a rare Gli1+ progenitor cell population contributing to liver regeneration during chronic injury

Fig. 2

a Schematic diagram showing the strategy for labeling Gli1+HNF4α+ cells by a dual recombinase-mediated intersectional genetic approach. b Schematic illustration of the experimental design. c Immunostaining for tdTomato (red) and HNF4α (green) in representative liver sections after DDC-induced injury. Scale bars, 50 μm. d Schematic illustration of the experimental design. e Immunostaining for GFP (green), tdTomato (red) and EpCAM (white), HNF4α (white) or KRT19 (white) in liver sections from Gli1-CreERt2;Ai9 mice. PV portal vein. Scale bars, 50 μm. f Immunostaining for GFP (green) and tdTomato (red) in liver sections from Gli1-CreERt2;Ai9 mice after DDC-induced injury and AAV8-TBG-GFP viral particle injection. White arrowheads indicate hepatocyte morphology. Scale bar, 50 μm.

Back to article page