Fig. 2: The pancreas does not exhibit lineage transdifferentiation between ductal and acinar cells during physiology.
From: Use of a dual genetic system to decipher exocrine cell fate conversions in the adult pancreas
a Schematic diagram illustrating the experimental strategy for Tam-induced lineage tracing and analysis. b Whole-mount bright-field and fluorescent images for zsGreen and tdT expression in pancreas from Tnni3-Dre;CK19-CreER;IR1 mice after 12 months of tracing. c, d Immunostaining for tdT, zsGreen and Amylase (c) or CK19 (d) on pancreatic sections collected from indicated time points. Yellow arrowheads, tdT+ acinar cells. White arrowheads, zsGreen+ ductal cells. e Quantification of the percentages of acinar cells expressing tdT or zsGreen and ductal cells expressing tdT or zsGreen from Tnni3-Dre;CK19-CreER;IR1 mice at indicated time points. Data are means ± SEM; n = 5. f Schematic diagram illustrating that the pancreatic exocrine gland does not exhibit lineage transdifferentiation during homeostasis. Scale bars, yellow, 1 mm; white,100 μm. Each image is representative of 5 individual samples.
