Fig. 1: Coronary endothelial cells contribute to endocardial cells after genetic depletion of endocardium. | Cell Discovery

Fig. 1: Coronary endothelial cells contribute to endocardial cells after genetic depletion of endocardium.

From: Coronary vessels contribute to de novo endocardial cells in the endocardium-depleted heart

Fig. 1

a Schematic showing knock-in strategy for generating Npr3-tTA allele. b Whole-mount fluorescence image of P2 heart. c Immunostaining for tdT and VE-Cad on heart sections from (b). d Schematic showing knock-in strategy for generation of Cdh5-LSL-tdT-DTR allele. e Whole-mount fluorescence image of E10.5 embryos. f, g Immunostaining for tdT and VE-Cad (f) or DTR (g) on E10.5 Cdh5-tdT-DTR;Actb-Cre embryonic sections. h Schematic showing genetic ablation of endocardium-derived endothelial cells. i Schematic showing the experimental strategy. j Whole-mount fluorescence image of P5 heart from Npr3-tTA;TetO-Cre;Cdh5-LSL-tdT-DTR mice treated with or without DT. k, l Immunostaining for tdT and CD31 on P5 heart sections treated without DT (k) or with DT (l). Arrowheads indicate connection between coronary vessels and the endocardium. m Schematic showing the experimental strategy for simultaneous coronary vessel tracing and endocardial cell depletion. n Immunostaining for GFP and CD31 on P14 heart sections treated with DT. Arrowheads, GFP+CD31+ cells. Right panel shows the quantification of the percentage of GFP+ endocardium. Data are means ± SEM; n = 8. o Immunostaining for GFP and PLVAP on P14 heart sections treated with DT. Arrowheads, GFP+PLVAP+ cells. p Cartoon image showing coronary endothelial cells convert to endocardial progenitors after depletion of endocardium-derived cells. Scale bar 1 mm (yellow), 100 µm (white).

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