Fig. 1: Preparation of bovine OGDC and PDC from native tissue lysate. | Cell Discovery

Fig. 1: Preparation of bovine OGDC and PDC from native tissue lysate.

From: Structures and comparison of endogenous 2-oxoglutarate and pyruvate dehydrogenase complexes from bovine kidney

Fig. 1

a Organization of E2 and E3BP domains. b Workflow for extraction of PDC and OGDC from bovine tissue lysate. Bovine kidney was homogenized to isolate mitochondria. Mitochondrial lysates were then fractionated by sucrose gradient. Samples were evaluated for α-keto acid dehydrogenase complex by SDS-PAGE, negative stain, and mass spectrometry. Cryo-EM reconstruction of these samples yielded icosahedral and cubic cores surrounded by peripheral densities.

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