Fig. 1: Cryo-EM structure of the EGF-bound EGFR/HER2 ectodomain complex. | Cell Discovery

Fig. 1: Cryo-EM structure of the EGF-bound EGFR/HER2 ectodomain complex.

From: Structure and dynamics of the EGFR/HER2 heterodimer

Fig. 1

a, b Detection of the EGFR/HER2 dimer using FSEC. GFP- and mCherry-tags are attached to the C-termini of EGFR and HER2, respectively. HER2_DT is the tail-deleted form with a substituted MBP-tag. EGFR_JM and HER2_JM constructs only contain the ectodomain, transmembrane domain, part of the juxtamembrane domain, and the coiled-coil (CC) peptide. The basic and acidic CC peptides are attached to EGFR_JM and HER2_JM, respectively. The red pentagram in b indicates the peak position of EGFR_JM/HER2_JM dimer. c Cryo-EM map of the EGF-bound EGFR/HER2 ectodomain complex shown in two views. EGFR, EGF, and HER2 are colored in green, marine, and magenta, respectively. d Overall structure of the EGF-bound EGFR/HER2 heterodimer in ribbon presentation. The color code is the same as that in c. The four domains of EGFR and HER2 ectodomains are indicated with Roman numerals. The DAs of Domain II are also labeled.

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