Fig. 7: mIHC staining showing clinical relevance and cellular crosstalk landscape for OSCC initiation. | Cell Discovery

Fig. 7: mIHC staining showing clinical relevance and cellular crosstalk landscape for OSCC initiation.

From: Single-cell and spatial dissection of precancerous lesions underlying the initiation process of oral squamous cell carcinoma

Fig. 7

a, b mIHC staining of two groups of selected markers (Group 1: PD-L1, TGFβ1, VEGFA, CD68, and PanCK; Group 2: APOE, CD68, FOXP3, OX40, and PanCK) in the samples of de novo OLK (a) and recurrent OSCC with OLK (b). Those recurrent OSCC samples with recurrent OLK were taken from patients who had OLK-derived OSCC before. The patients were under a clinical trial focusing on anti-PD-1 antibody treatment. Scale bars: 200 μm. Statistical quantification is shown in each image. c Flowchart showing the induction of OSCC by 4NQO in C57BL/6 mice and intraperitoneal injection of anti-PD-1/anti-TGFβ/anti-PD-1+ anti-TGFβ antibody in each treatment group. d Representative intraoral lesions on the tongues of mice in each group. e Macroscopic lesions on the tongues of each group. The dotted circle indicates cauliflower-like lesions. f Statistical results for quantification of the macroscopic cauliflower-like lesions in each treated and untreated group. A two-tailed Student’s t-test for the P values. *P < 0.05. g Potential maps on malignant transformation of epithelial cells and dynamic crosstalk between epithelial cells and the TMEs during OSCC initiation.

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