Fig. 5: Ubiquitination-mediated regulation is common in calpain family. | Cell Discovery

Fig. 5: Ubiquitination-mediated regulation is common in calpain family.

From: Calpain activity is negatively regulated by a KCTD7–Cullin-3 complex via non-degradative ubiquitination

Fig. 5

a, b Ubiquitous expressing calpains were expressed in HEK293 cells and α-spectrin cleavage was evaluated by immunoblotting, n = 3. c In vivo ubiquitination assay for calpains performed by coexpressing with HA-Ub in HEK293 cells for 36 h. d Co-IP analysis of Flag-tagged calpains and myc-tagged Cullin-3 in HEK293 cells. e Co-IP analysis of Flag-tagged calpains and myc-tagged KCTD7 in HEK293 cells. f In vivo ubiquitination assay in HEK293 cells for calpains performed by expressing alone or in combination with Cullin-3. g In vivo ubiquitination assay in HEK293 cells for calpains performed by expressing alone or in combination with KCTD7.

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