Fig. 6: KCTD7 regulates calpain activity in vivo. | Cell Discovery

Fig. 6: KCTD7 regulates calpain activity in vivo.

From: Calpain activity is negatively regulated by a KCTD7–Cullin-3 complex via non-degradative ubiquitination

Fig. 6

a, b Confocal microscopy analysis of cerebellar tissue from 5-week-old WT and Kctd7–/– mice showing CAPNS1 co-localization with the lysosomal marker LAMP1 in Purkinje cells. Trace outline is used for line-scan (white dashed line) analysis of relative fluorescence intensity of CAPNS1 and LAMP1 signals. Scale bar, 20 μm. c–e Cerebellar tissues from 5-week-old WT and Kctd7–/– mice were lysed in RIPA buffer and immunoblotted with the indicated antibodies, n = 4. f–l, Cerebellar tissues from 5-week-old WT and Kctd7–/– mice were lysed in NP-40 lysis buffer and calpain 1 and CAPNS1 were immunoprecipitated and probed for interaction with α-spectrin by immunoblotting (f–h). Lysates from same tissue samples were also immunoblotted for α-spectrin (f–i) and p35 (j–l) to evaluate calpain-mediated cleavage, n = 4. Data represent means ± SEM; ∗P < 0.05, ∗∗P < 0.01, ns not significant.

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