Fig. 1: The schematic diagram of phase separation studies using PROTAC.
From: BRD4-targeting PROTAC as a unique tool to study biomolecular condensates
PROTAC is believed to offer a new method for dynamic regulation in vivo and in vitro since it can regulate target proteins quickly, reversibly, effectively, and conveniently. The quick and reversible nature of PROTACs enables dynamic monitoring over a shorter time and allows us to detect phenomena that cannot be observed by genetic techniques like CRISPR-Cas9 or RNAi without being impacted by compensating effects following genetic alteration.
