Fig. 2: Establishment of the “PROTAC-target protein-LLPS” method.
From: BRD4-targeting PROTAC as a unique tool to study biomolecular condensates
a Chemical structure of ZXH-3-26. b Western blot analysis of BRD4 in HeLa cells treated with different concentrations of ZXH-3-26 for 6 h. c Joint analysis of BRD4 protein levels and condensate numbers with different concertation of ZXH-3-26 treatment (n = 30–35 cells). d Immunofluorescence was performed to track BRD4 condensates after different concentrations of ZXH-3-26 treatment for 6 h. Scale bars, 5 μM. e BRD4 was detected by western blot analysis at different time points after ZXH-3-26 treatment at 100 nM concentration. f Each time point sample was quantified for the average BRD4 condensate number by adding 100 nM ZXH-3-26. n = 30–35 cells per time point. g Immunofluorescence detection of BRD4 condensates at different time points treated with 100 nM ZXH-3-26. Scale bars, 5 μM.
