Fig. 3: Determination of phase separation properties of BRD4 using ZXH-3-26 treatment.
From: BRD4-targeting PROTAC as a unique tool to study biomolecular condensates
a Dynamic changes in BRD4 condensates with DMSO or ZXH-3-26 in EGFP-BRD4-expressing HeLa cells. Scale bars, 5 μM. b Statistical results of BRD4 condensate number and fluorescence intensity in EGFP-BRD4-expressing cells. c Western blotting was used to detect the protein level of BRD4 after the removal of ZXH-3-26 at different time points. d Immunofluorescence was used to track the BRD4 condensates after wash-out at different time points. Scale bars, 5 μM. e The number of BRD4 condensates per cell at different time points, n = 30–35 cells per time point.
