Fig. 4: The changes in the cellular genome and chromatin state caused by ZXH-3-26 treatment and wash-out experiments.

a The heat map of gene expression signatures of HeLa cells treated with ZXH-3-26 or wash-out experiments at different time points. b Compared with the DMSO group, the scatter diagram of gene changes after treatment with ZXH-3-26 for 6 h, wash-out 18 h, and wash-out 42 h. c GO analysis of significant genes at BRD4 degradation for 6 h and at different time points of wash-out experiments. d Heat map of BRD4 levels at transcriptional start sites (TSS) and enhancer after treatment with 6 h ZXH-3-26 (red), wash-out 18 h (green), wash-out 42 h (blue), or DMSO as vehicle control (gray). Each row shows ±3 kb centered on the BRD4 peak. Ranked was based on DMSO. CUT&Tag-seq signal was color-scaled intensities and normalized by spike-in controls. e Changes in BRD4 binding in SE regions were analyzed by CUT&Tag-Seq. f, g, h The gene track of BRD4 in the SE-driven locus of MYC (f), CDK6 (g), and SMAD3 (h) after wash-out experiments.