Fig. 3: TET2 suppresses mTORC1 activation via urea cycle.
From: TET2 is required to suppress mTORC1 signaling through urea cycle with therapeutic potential
a Knockdown of ASS1 blocks TET2 deletion-induced phosphorylation of S6K, S6 and 4EBP1. b Knockdown of ASL blocks TET2 deletion-induced phosphorylation of S6K, S6 and 4EBP1. c Knockdown of ASL blocks TET2 deletion-induced upregulation of arginine level. n = 3 biologically independent samples per group. d Knockdown of ASL blocks TET2 deletion-induced increase in cell size. n = 3 biologically independent samples per group. e sgTET2 cells are more resistant to arginine starvation with higher mTORC1 activity. Long exp long exposure, Short exp short exposure. f sgTET2 cells are more resistant to arginine starvation with higher proliferation ability. n = 3 biologically independent samples per group. g Arginine supplementation reverses the suppressive effect of TET2 overexpression on mTORC1 in sgTET2 HepG2 cells.
