Fig. 2: REDIC1 is localized to recombination intermediates in meiotic prophase cells.
From: A novel recombination protein C12ORF40/REDIC1 is required for meiotic crossover formation
a Representative spread spermatocytes of 30 dpp WT mice stained with antibodies against SYCP3 (blue), REDIC1 (green), and SIX6OS1 (magenta). Lep, leptotene; E-Zyg, early zygotene; L-Zyg, late zygotene; E-Pach, early pachytene; M/L-Pach, mid- or late pachytene; Dip, diplotene. Scale bars: 10 μm. b Quantification of the number of REDIC1 foci on chromosome axes at the indicated substages from WT mice. n, the total number of cells analyzed from two animals. The bars represent mean ± SD. c Representative images of zygotene (like) spermatocyte spreads from adult WT, Six6os1–/–, Top6bl–/–, and Dmc1–/– mice stained for SYCP3 (magenta) and REDIC1 (green). The experiments were repeated two times with similar results. Scale bars: 10 μm. d Representative images of surface-spread chromosomes from zygotene and pachytene mouse spermatocytes stained for RPA2 (magenta), REDIC1 (green), and the chromosomal axis marker SYCP2 (blue). The areas in the white rectangles are enlarged. e Structured illumination microscopy of spread zygotene and pachytene spermatocytes of Redic1-Flag/Myc mouse stained for MSH4 (magenta), Flag (green), and chromosomal axis marker SYCP2 (blue). The areas in the white rectangles are enlarged. The panel on the far right shows the magenta and green signals shifted by 8 pixels. Images are representatives from experiments with two adult animals. f Representative images of surface-spread chromosomes from a mid-pachytene spermatocyte stained for MLH1 (magenta), REDIC1 (green), and SYCP2 (blue) in WT mice. The magnified panels show a pair of synapsed chromosomes on which two MLH1 foci colocalized with two REDIC1 signals. The experiments were performed at least twice with similar results. Scale bars in d, e, and f are 10 μm for the original images and 2 μm for the enlarged images, respectively.
