Fig. 1: Structural characterization of the PaFtsWIQLB complex.
From: Structural insights into the activation of the divisome complex FtsWIQLB
a Schematic diagram of peptidoglycan synthesis by the divisome complex FtsWIQLB. b Topology of FtsW, FtsI, FtsL, FtsB, and FtsQ. c, d Cryo-EM map (c) and structure (d) of the PaFtsWIQLB complex. Flexible loops are shown as dashed lines. e Transmembrane helices of FtsW from the side and top views, showing ECL4 (grey). f The conserved residues present in the central cavity of FstW (corresponding residues in E. coli are in parentheses). g Cell viability and microscopic phenotypes of FtsW variants with mutated cavity residues. h Structural superimposition of PaFtsW-FtsI and TtRodA-PBP2 (PDB:6PL5) with overlapped FtsW and RodA as reference. i The interactions between FtsW and FtsL. j The putative catalytic residue D275 of FtsW interacts with residues within the central cavity. k–n Interactions between components of the FtsWIQLB complex are highlighted in boxes. Hydrogen bonds and salt bridges are indicated with yellow or black dashed lines.
