Fig. 3: Spatial separation of GC lineages and characterization of sublineages.

a Gene expression patterns of BARHL1 and TLX3 in GC lineages (top). Volcano plot showing the complementarily expressed gene cohorts (bottom). Symbols of almost evenly expressed genes in each subtype are colored blue (anterior) or green (posterior). b Spatial gene expression patterns of BARHL1 in anterior regions and TLX3 in posterior regions in both GW13 (top) and GW16 (bottom). SFRP1 labeled GC progenitors. c Schematic overview of three GC sublineages corresponding to three lobes in the human cerebellum. d Spatial organizations of GC sublineages in EGL and IGL. Genes detected in both EGL and IGL (top). Genes (the first gene corresponds to the outside one) enriched in EGL of each lobe (middle). Genes enriched in IGL of each lobe (bottom). e In situ hybridization results of Tlx3 and Parm1 in mouse at E15.5 and E18.5 adopted from Allen Brain (https://developingmouse.brain-map.org). Arrowheads indicated the detection of Parm1 in anterior but not in posterior lobes of mouse. f Correlation of human (left) or mouse (right) gene cohorts in human and mouse datasets showing species-specific spatial gene expression patterns. In each panel the bottom-left parts are human datasets. Only G1 phase GC lineage cells were calculated. g GRNs showing the potential regulatory programs of anterior (blue) or posterior (green) specific genes (top left) and visualization of accessible regions in different genes (bottom right). h Visualization of four subtypes of GC progenitor cells in scRNA-seq data and the expression of their marker genes (top left). Spatial distributions of PRR35, EBF2 and HEY1 in oEGL of GW13 (top right). Spatial distributions of HEY1, PRR35, EBF2 and GALNTL6 in EGL of GW16 (bottom).