Fig. 2: The unique assembly between the extracellular region of QRFPR and the N-terminus of 26RFa.
From: Structural basis for recognition of 26RFa by the pyroglutamylated RFamide peptide receptor
a The architecture of the extracellular region of QRFRP bound to the N-terminus of 26RFa. NT, N-terminus. b Effects of mutations of the extracellular region of QRFPR on the potency of 26RFa-induced calcium mobilization. pEC50 values are shown as means ± SEM from three independent experiments performed in triplicate. ***P < 0.001. NA not activated. All data were analyzed by two-sided, one-way analysis of variance (ANOVA) with Tukey’s test. c Structure comparison of the extracellular region of QRFPR with those of other class A peptide-activated GPCRs. The orientation of ECL2 in QRFPR relative to other class A GPCRs is depicted by a black arrow. GPRP gastrin-releasing peptide receptor (PDB: 7W40), GALR2 galanin receptor 2 (PDB: 7WQ4), CCKAR cholecystokinin A receptor (PDB: 7EZH), C5AR1 complement component 5a receptor 1 (PDB: 7Y65), BDKRB2 bradykinin receptor B2 (PDB: 7F2O).
