Fig. 4: IFN-α2a renders human neutrophil more immune-regulatory phenotype. | Cell Discovery

Fig. 4: IFN-α2a renders human neutrophil more immune-regulatory phenotype.

From: Sex-specific circulating unconventional neutrophils determine immunological outcome of auto-inflammatory Behçet’s uveitis

Fig. 4

a UMAP visualization of 17,270 neutrophils from BU patients (n = 18) and HCs (n = 16). b Heatmap showing relative expression of genes significantly (FDR < 0.05) covaried with pseudotime. c Cluster distribution density along pseudotime. d Trajectory analysis of peripheral neutrophils captures a pseudotime progression. e Schematic representation of the experimental design for co-culture experiments. f mRNA expression of MX1 and ANXA1 in the peripheral neutrophils from healthy individuals (n = 17) after treatment with/without IFN-α2a. g Correlation of MX1 and ANXA1 mRNA expression of peripheral neutrophils from healthy individuals (n = 17). h Effects of IFN-α2a pre-treated neutrophils on the frequencies of Th1/Th17 cells and Tregs in CD4+ T cells from active BU patients (female: n = 4; male n = 3) and healthy individuals (female: n = 5; male: n = 4). Data are shown as means ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. P-values were calculated by the Wilcoxon matched pairs test. In all instances, n refers to the number of each group.

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