Fig. 2: Myeloid lineage differentiation and TIME spatial location in SCLC. | Cell Discovery

Fig. 2: Myeloid lineage differentiation and TIME spatial location in SCLC.

From: Single-cell and spatial proteo-transcriptomic profiling reveals immune infiltration heterogeneity associated with neuroendocrine features in small cell lung cancer

Fig. 2

a DDRtree clustering of the Mono/Macro cells with DEGs selected using unsupervised methods implemented in monocle R package. b Representative immunofluorescence images showing the CD11c+ monocytes and CD68+ macrophages in the tumor vs stroma ROIs (based on SMA signal). c Heatmap visualizing genes that are differentially expressed in Mono/Macro cells from PBMC-enriched trajectories vs tissue-interacting trajectory. d Enriched gene ontologies of the DEGs in c. e CCI scores of each cell type pair based on the curated list of ligand–receptor (LR) pairs. Agglomerative hierarchical clustering was performed on a dissimilarity-like metric by taking the complement (1-score) of CCI scores, disregarding autocrine interactions. Cell types are colored by their lineages, as indicated in the legend. f Kaplan–Meier survival curves of 44 LS-SCLC patients based on the CD4 expression (stratified by median) assessed by IHC on whole tissue slides.

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